problems with primer dimer!

Peter Guo yuyuanguo at students.wisc.edu
Wed Sep 5 00:37:27 EST 2001


Hi,

Before using, melt the primers at 80 degree centigree. Or increase the mount
of template for double.

Peter
Babett Bartling <babett.bartling at medizin.uni-halle.de> wrote in message
news:3B8A4C6B.3DEC2735 at medizin.uni-halle.de...
> Hope somebody can help me.
> We investigated gene expression using PCR without any problems.
> Since last week, we suddenly have a lot of primer dimers and problems
> with the right size band.
> How can we solve the problem with the excess of primer dimers??
>
> Thanks in advance
>
> Andreas
>
>
>





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