SD consensus

Matthias Pfeiffer pfeiffer at
Wed Sep 5 07:14:04 EST 2001

Dear Trond,
it is well accepted that the 3' end of 16S rRNA is directly involved in the
procaryotic translation initiation by pairing with the Shine-Dalgarno (SD)
sequence, which is located in the ribosome-binding site of mRNA.
The consensus sequence GGAGGT  according to [1] [2]  is located 5 to 9 bases
upstream of the initiation codon, eg. GGAGGTAATATG
[1] Shine, J. and Dalgarno, L. Proc Natl Acad Sci USA 71, 1342-1346 (1974) 
[2] Gold, L. et al. Ann Rev Microb 35, 365-403 (1981)
Hope this helps,

Dr. Matthias Pfeiffer
Product Manager - Gene Synthesis

Thermo Hybaid GmbH
Sedanstr. 10, 89077 Ulm, Germany
tel +49-731-93579-290
fax +49-731-93579-291


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