help about the GST fusion protein

fly fly1234 at yeah.net
Wed Sep 5 09:58:39 EST 2001


hi
i used pGEX-2T vector to express a fusion protein with BL21. however, the
fusion protein (soluble ) could not be cleaved with excess thrombin (AP)
overnight at 22 degree in 1XPBS. the fusion protein was purified with
glutathione microspin columes (AP) and eluted in the elution buffer. the
thrombin we added was active because it could make plasma clot. i appreciate
any comments.

fei xie MD
research labs
center for lab medicine
fuzhou general hospital
China
Tel & Fax :86-591-3732129
feixie at chinalabmed.org




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