Copy number calculation

WeiHong Liu weihong.liu at adelaide.edu.au
Wed Sep 5 21:21:06 EST 2001


Dear there, 
I would like to construct a standard curve for absolute quantitation of
real-time PCR. I have a 180 and 230 bp purified DNA fragments with known
concentration, is anybody there knowing how to calculate the DNA copy
numbers according to the concentration and DNA fragment length? I have
searched Network but not found the answer. 
I will appreciate the help from all of you.
Cheers,
William W. Liu,
Department of Physiology,
Adelaide University, SA, Adelaide, 
Australia




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