mutagenesis kit

Cornelius Krasel krasel at
Thu Sep 6 01:01:51 EST 2001

Michael Witty <mw132 at> wrote:
> Kevin Brady wrote:
>> I used the Transformer kit from Clontech during my PhD, and I have to
>> say, there are easier ways of producing your mutants. I would recommend
>> using PCR mutagenesis, as the search for mutants is a little less
>> pain-staking.
> I would have thought that is true, but I have never tried these
> mutagenesis kits - does everyone think this?

Dependent on the type of mutagenesis I want to achieve, I use either
the Kunkel method (for point mutations) or PCR. I have made it a habit
to always introduce a new restriction site or remove an old one which
makes checking for mutants much less tedious.


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at */
/* "Science is the game we play with God to find out what His rules are."  */

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