fluorescent detection: microsatellites (help)

[Bruno Cenni]

...in cases where the PCR was really not working we added up to 10 ul of PCR
product. It worked, although I guess the salt load was so high that the
first injection didn't give as high peaks as later runs from the sample...

Bruno


"dbell" <dbell at qnis.net> wrote in message
news:01C136B7.B9296F20.dbell at qnis.net...

SNIP
> You can also vary the amount of
> PCR product you add to the diformamide, I have tried anywhere from 0.5ul
to
> 2.0ul.  This is a relatively easy gradient to try.
SNIP