labeling of mouse genomic DNA

Stéphane FLAMANT sflamant at pasteur.fr
Mon Sep 10 07:11:21 EST 2001


Hello,
I would like to radioactively (33P) label mouse genomic DNA in order to
hybridize blots. Could anyone give me a protocol for preparing the DNA
(I don't know what method I'd better use, between shearing, sonication,
or any other else...), the way to label it, and its purification before
use ?
Thanks a lot for your answers ; have a good day.

Stephane




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