bjm10 at cornell.edu
Mon Sep 10 07:33:58 EST 2001
"Balaji Kannan" <balajik at newton.berkeley.edu> wrote in
news:9nbr0j$2k2c$1 at agate.berkeley.edu:
> hi all
> is there anyone that knows how to denature double stranded DNA
> heating- i.e. using chemical methods?... or knows where to look for a
> protocol for that? i know that urea does it all the time in denaturing
> PAGE, but i want to do it in solution and with the correct protocol.
Bring the DNA solution to 0.1M NaOH and heat at 95 degrees C for 5 to 10
minutes. Plunge into ice. That's the old standard for Sanger sequencing.
"Cape Cod Salsa--somehow that's just not right."
More information about the Methods