freezing retrovirus supernatant

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Mon Sep 10 08:20:04 EST 2001


wolfsc at ibms.sinica.edu.tw ("Wolfgang Schechinger") wrote:
>Hi all, 
>
>a quick question, a quick answer is desired. I have some supernatant of 
>retrovirus producing cells). Is it possible to simply freeze the supernatant or
> 
>should I concentrate the virus (how? - this is another question) and add 50% 
>glyzerol.

If the volume is large (above ~ 10 ml), I would strongly recommend to 
concentrate first. This is done by simple ultracentrifugation. Then
resuspend in small volume and flash freeze in LN2. 50% glycerol
is a bad idea in this case IMHO. 

        - Dima




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