TA Clonning

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Mon Sep 10 09:06:57 EST 2001

In article <3B9CB1FC.E1018DF at pasteur.fr>, the eminent Stéphane FLAMANT
at Institut Pasteur, Paris wrote
>The classical Taq polymerase is
>suitable for such an aim.

Don't forget to run a 5-10min final extension at 72C to ensure overhangs
are there.

The other problem is that the sequence at the 5' end of trhe primers has
a big influence of whether an overhang (and percentage added) is added
or not - Biotechniques a couple of years ago.

The problem with the gene pool is that there is no lifeguard.

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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