freezing retrovirus supernatant
Dr. Duncan Clark
news at hgmp.mrc.ac.uk
Mon Sep 10 09:08:23 EST 2001
In article <3B9D25A0.19299.22FD7E8 at localhost>, the eminent Wolfgang
Schechinger at messy. wrote
>Is it possible to simply freeze the supernatant or
>should I concentrate the virus (how? - this is another question)
Not sure for mammalian virus, but 10% PEG6000-8000 with 0.5M NaCl (or is
it 1M) is pretty standard fort M13's and lambda etc.
The problem with the gene pool is that there is no lifeguard.
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