freezing retrovirus supernatant

Dr. Duncan Clark news at
Mon Sep 10 09:08:23 EST 2001

In article <3B9D25A0.19299.22FD7E8 at localhost>, the eminent Wolfgang
Schechinger at messy. wrote
>Is it possible to simply freeze the supernatant or 
>should I concentrate the virus (how? - this is another question)

Not sure for mammalian virus, but 10% PEG6000-8000 with 0.5M NaCl (or is
it 1M) is pretty standard fort M13's and lambda etc.

The problem with the gene pool is that there is no lifeguard.

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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