freezing retrovirus supernatant

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Mon Sep 10 09:08:23 EST 2001


In article <3B9D25A0.19299.22FD7E8 at localhost>, the eminent Wolfgang
Schechinger at messy. wrote
>Is it possible to simply freeze the supernatant or 
>should I concentrate the virus (how? - this is another question)

Not sure for mammalian virus, but 10% PEG6000-8000 with 0.5M NaCl (or is
it 1M) is pretty standard fort M13's and lambda etc.

Duncan 
-- 
The problem with the gene pool is that there is no lifeguard.

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382




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