TA Clonning

Bruno Cenni bruno.removethis.cenni at removethis.insel.ch
Mon Sep 10 10:50:45 EST 2001


As Duncan points out, the sequence of the primer influences the polymerase
(usually a problem in genotyping). I just had some refs on my desk:

Magnuson VL et al 1996 Biotechniques 21:700-9
Brownstein MJ et al 1996 Biotechniques 20:1004-10

Bruno


"Dr. Duncan Clark" <news@[127.0.0.1]> wrote in message
news:xzgSJMABkMn7EAYP at genesys.demon.co.uk...
> In article <3B9CB1FC.E1018DF at pasteur.fr>, the eminent Stéphane FLAMANT
> at Institut Pasteur, Paris wrote
> >The classical Taq polymerase is
> >suitable for such an aim.
>
> Don't forget to run a 5-10min final extension at 72C to ensure overhangs
> are there.
>
> The other problem is that the sequence at the 5' end of trhe primers has
> a big influence of whether an overhang (and percentage added) is added
> or not - Biotechniques a couple of years ago.
>
> Duncan
> --
> The problem with the gene pool is that there is no lifeguard.
>
> Duncan Clark
> GeneSys Ltd.
> Tel: +44(0)1252376288
> FAX: +44(0)8701640382





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