p53-disappearing supershifts?

Fernando Rodríguez Pascual frodriguez at cib.csic.es
Tue Sep 11 03:56:26 EST 2001


Hi,

As far as I know (I am not a gel shift expert) there are some antibodies
that supershift and other that just make the complex to disappear, but
this still demonstrates that your binding is specific and also the
effect of the antibody. I do not know the reason for that, maybe there
is anybody outside that knows why.

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______________________________________________________

Dr. Fernando Rodríguez Pascual
Centro de Investigaciones Biológicas (CIB)
Consejo Superior de Investigaciones Científicas (CSIC)
Velázquez 144, Madrid
E-28006 Spain
Phone 34 91 561 18 00 Ext. 4419
Fax 34 91 562 75 18
email: frodriguez at cib.csic.es
______________________________________________________


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