Transformation of a PCR-derived plasmid

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Tue Sep 11 04:05:24 EST 2001


In article <000801c13a93$511ad100$581380d4 at genetica>, the eminent
Joaquin Royo at BIOSCI/MRC Human Genome Mapping Project Resource Centre
wrote
>    Will there be problems transforming E. coli with plasmid DNA coming 
>    from PCR and, consequently, not methylated?. 

No.

>What is the E. coli 
>    strain of choice for that transformation?. 

Pick an hsdr-m- host, basically anything like XL1Blue, DH5alpha, TOP10
etc etc.

Duncan
-- 
The problem with the gene pool is that there is no lifeguard.

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382




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