Transformation of a PCR-derived plasmid
Dr. Duncan Clark
news at hgmp.mrc.ac.uk
Tue Sep 11 04:05:24 EST 2001
In article <000801c13a93$511ad100$581380d4 at genetica>, the eminent
Joaquin Royo at BIOSCI/MRC Human Genome Mapping Project Resource Centre
> Will there be problems transforming E. coli with plasmid DNA coming
> from PCR and, consequently, not methylated?.
>What is the E. coli
> strain of choice for that transformation?.
Pick an hsdr-m- host, basically anything like XL1Blue, DH5alpha, TOP10
The problem with the gene pool is that there is no lifeguard.
More information about the Methods