freezing retrovirus supernatant

[Victor L.]

It all depends on the envelope. In my hands ecotropic virus can
withstand one freezing (better-flash in liqN2) and thawing at 37^C
after storage at -80^C; you don't need to add anything.  Even so, the
titer drops ~4 fold. With VSVg pseudotyped titer stays pretty much the
same after one round.

Unlrafiltration helps but concentrating media proteins makes the final
product quite toxic <why?> to target cells and ecotropic virus was
destroyed <?> since the titer increase was insubstantial.

Your milage may vary....

Victor


On 10 Sep 2001 13:42:19 +0100, wolfsc at ibms.sinica.edu.tw ("Wolfgang
Schechinger") wrote:

>Hi all, 
>
>a quick question, a quick answer is desired. I have some supernatant of 
>retrovirus producing cells). Is it possible to simply freeze the supernatant or 
>should I concentrate the virus (how? - this is another question) and add 50% 
>glyzerol.
>
>Any ideas, recommendations ?
>
>Wo 
>[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
>-----
>Dr. Wolfgang Schechinger
>Lab N233 (c/o Dr. Steve Roffler)
>Institute of Biomedical Sciences
>Academia Sinica
>128 Yen-Chio Yuan Rd. Sec.2
>Taipei 115
>Taiwan R.O.C.
>Tel +886-2-2789-9152
>Fax +886-2-2782-9142
>Mobile +886-925-136893
>e mail wolfsc at ibms dot sinica dot edu dot tw
>-----
>
>---