Sequencing or Gene Specific Primer

Kunika kunika_jain at rediffmail.com
Wed Sep 12 04:35:25 EST 2001


Hello All, 
I would like to amplify the a gene from a cDNA clone as well the suspected
same gene from a another vector in which its been subcloned previously. Can
anybody suggest me either I should use the gene specific primer or can I use
the universal primers?

If suggestion goes towards the use of universal primers, how, if I useT3 and
T7 promoters?

Thank you...!

Kunika  


<http://www.biowww.net/forum/read.php?f=1&i=3797&t=3797>




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