lymphocyte cell culture

T. Patrick Purk purk at
Thu Sep 13 18:10:04 EST 2001


I am interested in making an immortalized lymphocytic cell line from a
knockout mouse.  My hope is to use this line for proteomic analysis
following viral gene replacement.  I apologize for this post if this is a
common procedure, but I am looking for a method that will minimally effect
background gene expression.

Thank you for your time,

T. Patrick Purk
Medical Scholars Program
University of Illinois, Urbana-Champaign
email: purk at

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