DNA/RNA-hybrid Cap-finder Oligo

Ricky Boernke ricky_boernke at gmx.net
Fri Sep 21 01:57:24 EST 2001


Dear all,

when looking at the protocol/literature concerning Clontech's SMART RACE
procedure I realized that the template switch oligo is having an
oligo(rG)
sequence at its 3' end. Is this really an DNA/RNA-hybrid oligo or is the
rG part derived from 2'OMe ribonucleotides. The thing is that I would
like
to synthezise a template switch oligo myself and the oligo company told
me
that they are not able to make real RNA, because of the  2'hydroxy group
of
the ribonucleotide.
Any input welcome
Ricky




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