DNA/RNA-hybrid Cap-finder Oligo

Ricky Boernke ricky_boernke at gmx.net
Fri Sep 21 01:57:24 EST 2001

Dear all,

when looking at the protocol/literature concerning Clontech's SMART RACE
procedure I realized that the template switch oligo is having an
sequence at its 3' end. Is this really an DNA/RNA-hybrid oligo or is the
rG part derived from 2'OMe ribonucleotides. The thing is that I would
to synthezise a template switch oligo myself and the oligo company told
that they are not able to make real RNA, because of the  2'hydroxy group
the ribonucleotide.
Any input welcome

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