Multiple plasmid FuGENE 6 transfections

DAVID JACKSON devilfish at
Fri Sep 21 13:40:49 EST 2001

12 plasmid transfections are not absurd.  I do these on a regular basis to
rescue flu A viruses from cloned cDNAs.  The system is a well-recognised
system for virus rescue (first described for flu in 1999 - Neumann et al.)
and is used all around the world.  My problem is in rescuing flu B viruses
(as yet not possible) and the constructs used in this case were produced in
different ways - hence my question.

In this case immunofluorescence is not required as the production of virus
particles and CPE is the proof of protein expression.  Maybe CaPO4
transfection is the answer.



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