Staph. aureus transformation

Tom Arnes u23s at hotmail.com
Mon Sep 24 06:57:01 EST 2001


Hi,
I'm trying to transform S. aureus with plasmid DNA.
Should I be aware of any differences compared to E. coli electroporations?
What settings would you recommend for the electroporation?
Any hints as to the choice of growth medium? The wild-type seems to be
growing fine on LB. Is there anything "better" you'd recommend (i.e.
increase the salt conc in the medium)?
Thanks,
Tom.






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