3 prime fill in reaction?

Peter Ashby p.r.ashby at dundee.MAPS.ac.uk
Wed Sep 26 11:12:15 EST 2001

A colleague wants to do a 3 prime fill in reaction on BsrD1 cut DNA. She 
has tried T4 polymerase (which works to fill in NcoI) but it appears to 
chew BsrD1 back. Is there any known way to fill in a 3 prime overhang? 
If not we will have to redesign everything :-(


Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
Reverse the spam and remove to email me.

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