3 prime fill in reaction?

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Sep 26 12:06:36 EST 2001


Dear Peter,
          doesn't BsrD1 have a 3' overhang?  This makes it one of the ends
you have to chew back rather than fill in (I think).  Maybe you need PCR
too help you out of this tight corner.  Regards, Mike.


On Wed, 26 Sep 2001, Peter Ashby wrote:

> A colleague wants to do a 3 prime fill in reaction on BsrD1 cut DNA. She
> has tried T4 polymerase (which works to fill in NcoI) but it appears to
> chew BsrD1 back. Is there any known way to fill in a 3 prime overhang?
> If not we will have to redesign everything :-(
>
> Peter
>
> --
> Peter Ashby
> Wellcome Trust Biocentre
> University of Dundee
> Dundee, Scotland
> Reverse the spam and remove to email me.
>




More information about the Methods mailing list