joining PCR fragments into a single product

Alex ayboro at my-deja.com
Thu Sep 27 10:31:46 EST 2001


Hi all,
I'm wondering if anybody can help me with a reference. I remember an
article (I think at was in Biotechniques) on a simple polymerase based
method that allows to generate a single fragment from two PCR
generated overlapping halfs. Combining products of 5' and 5' RACE into
one contiguous DNA fragment without restriction/ligation has been used
as an example.
Do anybody remember the article and can provide me with the reference.
Sincerely,
Alex




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