background on 2D western blot

Will Chiu will.chiu at
Fri Sep 28 05:31:51 EST 2001

Dear All
I have been experiencing intense spotty backgrounds on my 2D western blot
probed with sera using NC membrane. A switch to PVDF membrane managed to rid
of the spots but background noise was too great despite of trial of various
blocking agents.
would nylon membrane help?
Any suggestion is welcome.


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