background on 2D western blot
mw132 at mole.bio.cam.ac.uk
Fri Sep 28 06:38:35 EST 2001
to you mean pin-point spots or larger smeary spots? Mike.
On 28 Sep 2001, Will Chiu wrote:
> Dear All
> I have been experiencing intense spotty backgrounds on my 2D western blot
> probed with sera using NC membrane. A switch to PVDF membrane managed to rid
> of the spots but background noise was too great despite of trial of various
> blocking agents.
> would nylon membrane help?
> Any suggestion is welcome.
More information about the Methods