background on 2D western blot

Michael Witty mw132 at
Fri Sep 28 06:38:35 EST 2001

Dear Will,
         to you mean pin-point spots or larger smeary spots?  Mike.

On 28 Sep 2001, Will Chiu wrote:

> Dear All
> I have been experiencing intense spotty backgrounds on my 2D western blot
> probed with sera using NC membrane. A switch to PVDF membrane managed to rid
> of the spots but background noise was too great despite of trial of various
> blocking agents.
> would nylon membrane help?
> Any suggestion is welcome.
> Help
> <>

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