joining PCR fragments into a single product
cboyd at holyrood.ed.ac.uk
Fri Sep 28 06:12:32 EST 2001
Alex <ayboro at my-deja.com> wrote:
: Hi all,
: I'm wondering if anybody can help me with a reference. I remember an
: article (I think at was in Biotechniques) on a simple polymerase based
: method that allows to generate a single fragment from two PCR
: generated overlapping halfs. Combining products of 5' and 5' RACE into
: one contiguous DNA fragment without restriction/ligation has been used
: as an example.
: Do anybody remember the article and can provide me with the reference.
It's also worth looking at
1. Wallace, A. J., Wu, C. L. and Elles, R. G. (1999) `Meta-PCR: a
novel method for creating chimeric DNA molecules and increasing the
productivity of mutation scanning techniques.' Genet. Test., 3,
where an improved megaprimer method is described that allows catenation
of 2 or more PCR products.
Chris Boyd | from, but \ Medical Genetics Section
Chris.Boyd at ed.ac.uk | not for, / MMC, Edinburgh Uni.,
http://www.ed.ac.uk/~cboyd EH4 2XU, SCOTLAND
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