OSMOTIC SHOCK PROTOCOLS
mw132 at mole.bio.cam.ac.uk
Fri Sep 28 17:47:28 EST 2001
I used the one in the Qiagen Ni-NTA protein purification
booklet (on their web site), for E. coli, but I used it for Pseudomonas
aeruginosa. My preparation was very messy (with lysed cells) - however I
am not really sure how good periplasmic preps are supposed to be!
On 28 Sep 2001, Marcelo Foti wrote:
> Hi all, I need protocols of osmotic shock to find periplasmic protein
> expressed in E coli,
> thanks in advance.
> Marcelo Foti
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