OSMOTIC SHOCK PROTOCOLS

Michael Witty mw132 at mole.bio.cam.ac.uk
Fri Sep 28 17:47:28 EST 2001


Dear Marcelo,
            I used the one in the Qiagen Ni-NTA protein purification
booklet (on their web site), for E. coli, but I used it for Pseudomonas
aeruginosa.  My preparation was very messy (with lysed cells)  - however I
am not really sure how good periplasmic preps are supposed to be!
Regards, Mike.

 On 28 Sep 2001, Marcelo Foti wrote:

>
>  Hi all, I need protocols of osmotic shock to find periplasmic protein
> expressed in E coli,
>
>
> thanks in advance.
>
> Marcelo Foti
> Argentina
>
> ---
>




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