My pLys confusion

[Jeffery Haines]

Hello all,

Just wondering if someone can give me the "in a nutshell" summary of 
pLys.  We're using BL21 cells in the lab I work in, and I've always 
wondered the purpose of it.  I did the good ol' Google search and I got 
more confused... 1) encodes T7 lysozyme which inhibits T7 RNA poly (which 
I guess would be good when you're not expressing protein, but is it still 
expressed when expression is going on?  (is the lysozyme constantly 
expressed?).  2)  chops up peptidoglycan in E. coli membr.   
Is this pLysS thing doing both?  What is pLysE?  Can anyone give me the 
story or recommend a website that is not trying to advertise a product 
that is for sale? LOL

Cheers to all!
Jeff


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Jeff Haines
4th year undergrad
BSc Biochemistry