PCR questions...

NM acoustic52 at hotmail.com
Mon Apr 1 02:30:13 EST 2002

Two questions:

1) Will MgCl 'go off' after repeateded freeze/thaws??
2) Is it common to do a PCR without doing a hot start and see nothing,
but then get a great product using a hot start??? It seems unlikely
that it would go from nothing to something under these conditions...
by the way, the product size is 1100bp...

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