TCEP

Mariella parisi at fis.unipr.it
Tue Apr 2 11:14:46 EST 2002


Does anybody have experience with using tris-(2-carboxyethyl) phosphine (TCEP)
or other phosphines for reduction of disulfide bonds in proteins.

Is the reagent easy to handle?

What protocol do you use:
1. n(TCEP):n(protein -SS-)
2. temperature
3. time
4. pH
more?

Where can the reagent be bought

thank you
 Mariella




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