khatipovNO at NOuchicago.edu
Tue Apr 2 12:37:45 EST 2002
You can find all the necessary information on the Pierce webite
(www.piercenet.com - no affiliation). I recommend buying their neutral pH
TCEP solution (500mM) that is very convenient. Basically, you should use
this reagent at 50mM to reduce disulphides (~10-30 min). They also have a
great product that is TCEP immobilized to the resin, so that you don't need
to desalt your sample after reduction any longer.
"Mariella" <parisi at fis.unipr.it> wrote in message
news:fad5fa87.0204020814.36724da9 at posting.google.com...
> Does anybody have experience with using tris-(2-carboxyethyl) phosphine
> or other phosphines for reduction of disulfide bonds in proteins.
> Is the reagent easy to handle?
> What protocol do you use:
> 1. n(TCEP):n(protein -SS-)
> 2. temperature
> 3. time
> 4. pH
> Where can the reagent be bought
> thank you
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