H. influenzae RE digests early '70s...

Ned Mantei mantei at cell.biol.ethz.ch
Wed Apr 3 03:22:23 EST 2002


In article <3ca9e852$1 at news.uia.ac.be>,
 Roland Hübner <roland.hubner at ua.ac.be> wrote:

>Hello,
>
> in a paper from 1972 a RE digest of SV40 strains with H. influenza is 
>shown, but the 11 fragments that are displayed do not correspond with 
>HindIII or HincII or HinfI digest patterns... Perhaps a mixture ?
>
> Anybody happen to know whether a combo was frequently isolated from H. 
>influenzae those days...
>
It was "Hind", which is a combination of Hind III and Hind II. Hind II 
is the same as Hinc II (except that Hind II is isolated from a serotype 
d strain and Hinc II from serotype c). It was only later realized that 
H. influenza d contains more than one restriction enzyme (I don't know 
what happened to Hind I).

-- 
Ned Mantei
Department of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland




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