mutation rate in eukaryotic cultured cells

Wolfgang Schechinger wolfsc at
Wed Apr 3 10:59:55 EST 2002

Dear all, 

I have some 3t3 cells that are stable expressing a 2kbp cDNA (prepared by 
retroviral infection, the cell line was started from a single cell). They 
divide and grow happily and are split 1 : 50 every 2 or 3 days.

The gene in question is very likely not affecting survival (i.e. does not 
exert positive or negative selection pressure if mutations therein occure).

When I culture these cells for a long time, how high is the likeliness to get 
mutations in this gene? Is there any data available on the mutation rate one 
could assume? How heterogenuous could a population become (in respect to that 
gene) when the cells are kept in culture for say half year or longer?

All input is welcome.

Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan


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