mutation rate in eukaryotic cultured cells
wolfsc at ibms.sinica.edu.tw
Wed Apr 3 10:59:55 EST 2002
I have some 3t3 cells that are stable expressing a 2kbp cDNA (prepared by
retroviral infection, the cell line was started from a single cell). They
divide and grow happily and are split 1 : 50 every 2 or 3 days.
The gene in question is very likely not affecting survival (i.e. does not
exert positive or negative selection pressure if mutations therein occure).
When I culture these cells for a long time, how high is the likeliness to get
mutations in this gene? Is there any data available on the mutation rate one
could assume? How heterogenuous could a population become (in respect to that
gene) when the cells are kept in culture for say half year or longer?
All input is welcome.
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan
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