5' RACE Problem

Iwona iwonajeff at Yahoo.com
Fri Apr 5 15:50:53 EST 2002


Hello All,

Has anybody gotten good results with 5' RACE?  I have used Clontech Kit, and
my gene is ~100 bp short of 5' end.  I cannot get to the beginning of the
gene.  I know that it is very GC rich (~90% in that region), and I tried
adding GC melt to the RT- PCR reaction, but that does not help.  Any
suggestions?
 I did call Clontech and they did not have any ideas.
Thanks.
Iwona


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