Yeast gDNA construction
orangerieus at yahoo.com
Mon Apr 8 06:41:06 EST 2002
I am trying to contruct genomic library for yeast in Yep13.
I am using Promega Sau3A to do the partial digestion. The Yep13 is cut with
Roche BamHI and dephosporylated.
The problem I have is the ligations I had tried so far only managed to give
me not more than 400 colonies in total (50ng of vector is used, 1:1 molar
Wondering whether anyone there knows a solution to my problem. Thank you.
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