Yeast gDNA construction

hhh orangerieus at
Mon Apr 8 06:41:06 EST 2002


I am trying to contruct genomic library for yeast in Yep13.
I am using Promega Sau3A to do the partial digestion. The Yep13 is cut with 
Roche BamHI and dephosporylated.

The problem I have is the ligations I had tried so far only managed to give 
me not more than 400 colonies in total (50ng of vector is used, 1:1 molar 
ratio, electroporation).

Wondering whether anyone there knows a solution to my problem. Thank you.


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