RNA clean-up with silica-based columns

Tyson mediablitz at home.com
Tue Apr 9 11:22:17 EST 2002


Does anybody here have any experience with cleaning up extracted RNA using
Qiagen (or similar) RNeasy columns?

...I've tried doing it with 50ug of purified total RNA resuspended in EtOH,
following Qiagen's suggested clean-up protocol, but I get yields of
<50%....this of course is unacceptable.  Anybody have similar problems?

Thanks in advance,
Tyson





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