M shaped bands

me melectronica at hotmail.com
Wed Apr 10 14:40:17 EST 2002


Hi!
We have troubles with the detection of proteins of 12 - 25 kDa in 15%
SDS-PAGE and Western blots. The proteins comes from tissue extracts (aortas)
and we are using 100 - 200 µg of total protein per lane. The proteins of low
molecular weight appears as a smear and is impossible to differentiate any
band. In some cases this proteins appears as M shaped bands. Do you think
that the problem is the acrylamide or there is any other problem in the gel
or in the sample.
Thanks!


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