M shaped bands

ChenHA hzhen at freeuk.com
Thu Apr 11 04:29:27 EST 2002

me wrote:
> Hi!
> We have troubles with the detection of proteins of 12 - 25 kDa in 15%
> SDS-PAGE and Western blots. The proteins comes from tissue extracts (aortas)
> and we are using 100 - 200 µg of total protein per lane. The proteins of low
> molecular weight appears as a smear and is impossible to differentiate any
> band. In some cases this proteins appears as M shaped bands. Do you think
> that the problem is the acrylamide or there is any other problem in the gel
> or in the sample.
> Thanks!

Try tricine SDS-PAGE gel if you want to detect lower mw proteins.

> <http://www.biowww.net/forum/read.php?f=6&i=993&t=993>

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