keeping adherent cells ins suspension culture?
wolfsc at ibms.sinica.edu.tw
Thu Apr 11 05:13:12 EST 2002
How can I overcome this problem:
I have cells expressing an enzyme pinned on the extracellular part of the
membrane. To assay this enzyme by FACS, I need to trypsinize the cells, but
this (just 5 min) reduces the enzyme activity significantly. Detaching the
cells with EDTA kills too many of them.
What can I do to either
1) obtain a single cell suspension or
2) to keep the cells for some time in suspension until some newly synthesized
protein is delivered to the membrane?
I welcome your input
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan
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