DNA purification loss, large insert

Duncan Clark junk at hgmp.mrc.ac.uk
Mon Apr 15 05:37:21 EST 2002


Historians believe that in newspost 
<1NJt8.66228$VM5.24781131 at e3500-atl1.usenetserver.com> on Fri, 12 Apr 
2002, Gr8Guy <ct4fren*ch at bell&sou7th.n8et> penned the following literary 
masterpiece:
>I'm considering ligating them in the gel slices, using low melting agarose.
>Does anyone have a reliable method for extracting DNA frags over 5kb that
>gives an acceptable recovery yield?

Low melting point agarose and Agarase enzyme. Supposedly good for BACs 
etc.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.




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