DNA purification loss, large insert

Duncan Clark junk at hgmp.mrc.ac.uk
Mon Apr 15 05:37:21 EST 2002

Historians believe that in newspost 
<1NJt8.66228$VM5.24781131 at e3500-atl1.usenetserver.com> on Fri, 12 Apr 
2002, Gr8Guy <ct4fren*ch at bell&sou7th.n8et> penned the following literary 
>I'm considering ligating them in the gel slices, using low melting agarose.
>Does anyone have a reliable method for extracting DNA frags over 5kb that
>gives an acceptable recovery yield?

Low melting point agarose and Agarase enzyme. Supposedly good for BACs 

The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.

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