keeping adherent cells ins suspension culture?

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Mon Apr 15 09:05:56 EST 2002


Frank O. Fackelmayer <Frank at fackelmayer.de> wrote:
> Wolfgang Schechinger wrote:

[about keeping cells in suspension]

>> 2) to keep the cells for some time in suspension until some newly synthesized
>> protein is delivered to the membrane?
> 
> Try to keep the cells in PBS with 2mM pyruvate

Something that has only partially to do with the original question:
why do you use pyruvate and not, for example, 10 mM glucose?

Curious,
--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at wpxx02.toxi.uni-wuerzburg.de */
/* "Science is the game we play with God to find out what His rules are."  */




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