TME at biomedicaldesign.com
Mon Apr 15 11:50:38 EST 2002
I am trying to adapt the MTT viability assay for use in 6 well plates
(as opposed to the 96 well plates that the protocols are written for).
I have a few questions that I hope you can help me with.
1: After solubilizing, is the formazan released into the medium or is
it maintained in the cells? (ie: Can the medium be removed and read
in a cuvette style spectrometer?).
2: Are there any references to this assay being accurate for 3x10^5
cells or more per well? If so, could you please point in the right
direction to find this reference.
3: An old laboratory protocol I found calls for using Hanks BSS for
the incubation step, yet I have found 1 report that using BSS's are
not ideal? Any thoughts/experience with this?
4: Does anyone know of a protocol where this assay has been modified
for use in other than 96 well plates?
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