EMSA with cell from mouse embryos

Nadine Landgraf nadine.landgraf at hll.de
Wed Apr 17 07:38:02 EST 2002


Hi,
I want to do gel-shifts with cells that I earn from mouse embryos day
10,5 p.c.. It seems to be impossible to cultivate these cells so I
want to use cell extracts directly from freshly prepared embryos. Has
anybody done an EMSA with not-cultivated cells? How many cells or
embryos (without head) will be needed? Is there a possibility to get a
protocol? (I am trying to do a promoter analysis of dll1.)

Thank you for helping me!

Nadine




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