Methods for apoptotic mechanisms' investigation

[stéphane califice]

We work with a prostate cancer cell line (LNCaP) that doesn't express 
our protein of interest. We have obtained clones that express that 
protein only in the cytoplasm, and others that express it only in the 
nucleus. The cytoplasmic clones show a decreased apoptosis compared 
to the parental cell line and control mock-transfected clones. This 
has been shown in other cell lines and explained by interaction 
between that protein and bcl-2.

At the opposite, the nuclear clones show an increased apoptosis 
compared to the parental cell line and control mock-transfected 
clones. We are looking for methods to investigate by which mechanisms 
the expression of our protein of interest in the nucleus could 
promote apoptosis. If you have any idea, please reply at this address 
: scalifice at student.ulg.ac.be

Thank you very much.

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Stéphane Califice

Metastasis Research Laboratory (MRL)
Tour de Pathologie B23/-1 Sart-Tilman
B-4000 LIEGE
BELGIUM
Phone: 32(0)4 366.25.56

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