HEPES and calcium phosphate

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Apr 17 22:43:35 EST 2002


On 18 Apr 2002 04:35:57 +0100, wolfsc at ibms.sinica.edu.tw ("Wolfgang
Schechinger") wrote:

>Dear all, 
>
>I wonder if HEPES in some media formulations interferes with calcium phospahte 
>based transfection protocols.
>

It shouldn't. It's not metal-chelating, and also ISTR using a CaPhos
based transfection protocol back in the 80's that used HEPES buffered
saline as a diluent.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu




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