how to induce site-directed mutation?

Wolfgang Schechinger wolfsc at
Thu Apr 18 11:52:00 EST 2002


It also works without kits. Just have DpnI (eg from NEB) and Pfu or Pwo 
(and primers and a thermocycler of course).




> if your gene of interest is in a plasmid or something that PCR can run
> around, try Quickchange mutagenesis (stratagene).  It's pretty easy and
> efficient.
> Good luck,
> -Jiab
> <>

Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan


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