how to induce site-directed mutation?

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Thu Apr 18 11:52:00 EST 2002


Hi,

It also works without kits. Just have DpnI (eg from NEB) and Pfu or Pwo 
(and primers and a thermocycler of course).

See:
http://ww.google.com/search?hl=en&q=site+directed+mutagenesis+pcr
http://www.nwfsc.noaa.gov/protocols/pcr-mut.html

Enjoy!

Wo 




> if your gene of interest is in a plasmid or something that PCR can run
> around, try Quickchange mutagenesis (stratagene).  It's pretty easy and
> efficient.
> 
> Good luck,
> 
> -Jiab
> 
> 
> <http://www.biowww.net/forum/read.php?f=1&i=5392&t=5372>
> 
> 


-----
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan

---




More information about the Methods mailing list