how to induce site-directed mutation?
wolfsc at ibms.sinica.edu.tw
Thu Apr 18 11:52:00 EST 2002
It also works without kits. Just have DpnI (eg from NEB) and Pfu or Pwo
(and primers and a thermocycler of course).
> if your gene of interest is in a plasmid or something that PCR can run
> around, try Quickchange mutagenesis (stratagene). It's pretty easy and
> Good luck,
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan
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