Isolation of periplasmic proteins

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Wed Apr 24 08:24:27 EST 2002


You might check out the pET manual at novagen's web site 
(www.novagen.com).  Since they sell some vectors that direct 
expression to the periplasmic space, I do believe they provide a 
protocol (and some references) for getting such proteins out.

Mike

>Dear colleagues,
>
>Do you know of a protocol to isolate proteins from periplasm and not 
>get contamination from cytoplasm?
>
>Trond Erik Vee Aune

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