Isolation of periplasmic proteins

Michael Witty mw132 at mole.bio.cam.ac.uk
Thu Apr 25 05:16:02 EST 2002


On 25 Apr 2002, Wolfgang Schechinger wrote:

> Dear Trond,
>
> In addition to Mike's response, there's also a product from NEB, pMAL-p2 with
> a manual worth checking.
>
> Regards,
>
> Wo
>
> > Dear colleagues,
> >
> > Do you know of a protocol to isolate proteins from periplasm and not get
> > contamination from cytoplasm?
> >
> > Trond Erik Vee Aune

. . . I am assuming all these are based on the osmotic shock: when I tried
this I did get a grubby prep with a lot of cytoplasmic protein.  Regards,
Mike.




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