Non-alpha complementation vector

Duncan Clark junk at
Thu Apr 25 08:14:06 EST 2002

Historians believe that in newspost 
<20020424171001.01655.00002368 at> on Wed, 24 Apr 2002, 
JSUPolek <jsupolek at> penned the following literary masterpiece:
>Does anyone know of a high copy number vector that does not contain the
>truncated lacZ gene?  I've been using pBR322

pHC624, pHC312, pHC314.

Single point mutation of a pBR322 derivative (it's a tet minus 
derivative as tet is lethal at high copy). Published many many years ago 
in Gene by a Hungarian group I think.

I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

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