Non-alpha complementation vector
junk at hgmp.mrc.ac.uk
Thu Apr 25 08:14:06 EST 2002
Historians believe that in newspost
<20020424171001.01655.00002368 at mb-cl.aol.com> on Wed, 24 Apr 2002,
JSUPolek <jsupolek at aol.com> penned the following literary masterpiece:
>Does anyone know of a high copy number vector that does not contain the
>truncated lacZ gene? I've been using pBR322
pHC624, pHC312, pHC314.
Single point mutation of a pBR322 derivative (it's a tet minus
derivative as tet is lethal at high copy). Published many many years ago
in Gene by a Hungarian group I think.
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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