Any protocol for Gi protein extraction from rat brain?
krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Apr 26 13:05:40 EST 2002
Ning LI <libra at ion.ac.cn> wrote:
> We need to extract Gi protein. Does anybody have protocol for that?
1 ) Is Gi the inhibitory G-protein?
1a) If yes, do you need the heterotrimer or only the alpha subunit?
2 ) What is your starting material (i.e. do you want to produce
recombinant protein or do you want to do immunoblotting from
tissue or ...) ?
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
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